Skip to main navigation menu Skip to main content Skip to site footer

Original article

Vol. 142 No. 3940 (2012)

Cryptosporidium spp. in drinking water

  • Hans Peter Füchslin
  • Thomas Egli
  • Stefan Kötzsch
DOI
https://doi.org/10.4414/smw.2012.13683
Cite this as:
Swiss Med Wkly. 2012;142:w13683
Published
23.09.2012

Summary

In most rural areas and small communities in Switzerland the drinking water is supplied to the consumers after a minimum or even no treatment at all. However, it is just in these areas where drinking water from sources of agricultural activities can be contaminated by liquid manure and faeces of pasturing animals. The Swiss drinking water regulations are limited to the monitoring of E. coli, Enterococcus spp. and total plate counts only. Hence, resistant pathogens, as for example Cryptosporidium spp., remain unnoticed. During a drinking water survey, which lasted from June 2003 to December 2004, water samples were collected from 3 selected rural sites in Switzerland. The drinking water was investigated for Cryptosporidium spp., E. coli, Enterococcus spp., Clostridium perfringens and other parameters. In all samples oocysts of Cryptosporidiumspp. were detected at elevated concentrations of up to 0.18 oocysts/l. Between 28% and 75% of the oocysts were found to be vital by the excystation method. Sampled oocysts collected from the three sites were subjected to genotyping and in one case the isolate was found to belong to the genotype of C. parvum. No evidence for increased incidents of diarrhoea in the past years was noted by local authorities.

References

  1. Carey C. Biology, persistence and detection of Cryptosporidium parvum and Cryptosporidium hominis oocyst. Water Res. 2004;38:818–62.
  2. Juranek DD. Cryptosporidiosis: sources of infection and guidelines for prevention. Clin Infect Dis. 1995;21(Suppl 1):S57–61.
  3. DuPont HL, Chappell CL, Sterling CR, Okhuysen PC, Rose JB, Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. N Engl J Med. 1995;332(13):855–9.
  4. Campbell IS, Tzipori S, Hutchinson G, AK W. Effect of disinfectants on survival of Cryptosporidium oocysts. Vet Rec. 1982;111:414–5.
  5. Xiao L, Fayer R, Ryan U, Upton SJ. Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev. 2004;17(1):72–97.
  6. Peng MM, Xiao L, Freemaan AR, and al. e, Genetic polymorphism among Cryptosporidium parvum isolates: Evidence of two distinct human transmission cycles. Emerg Infect Dis. 1997;3:567–73.
  7. Smith HV, Rose JB. Waterborne cryptosporidiosis: current status. Parasitol Today. 1998;14(1):14–22.
  8. MacKenzie WR, Schell WL, Blair KA, Addiss DG, Peterson DE, Hoxie NJ, et al. Massive outbreak of waterborne Cryptosporidium infection in Milwaukee, Wisconsin: recurrence of illness and risk of secondary transmission. Clin Infect Dis. 1995;21(1):57–62.
  9. Hunter PR, Syed Q. Community surveys of self-reported diarrhoea can dramatically overestimate the size of outbreaks of waterborne cryptosporidiosis. Water Sci Technol. 2001;43(12):27–30.
  10. Haas CN, Rose JB. Developing an action level for Cryptosporidium. AWWA. 1995;87(9):81–3.
  11. USEPA, National Primary Drinking Water Regulations: filtration, disinfection, turbidity, Giardia lamblia, viruses, Legionella, and heterotrophic bacteria; Final Rule, in Federal Register (40 CFR Parts 141 and 142). 1989.
  12. Teunis P, Havelaar A. Risk assessment for protozoan parasites. Internat Biodeterior & Biodegr. 2002;50:185–93.
  13. Metzler A, Tabisch A. Fakten und Spekulationen über die Kontamination der Umwelt mit Cryptosporidium-Oozysten. Gas, Wasser, Abwasser (GWA), 1998;78(1):32–6.
  14. USEPA, National Primary Drinking Water Regulations: Long Term 2 Enhanced Surface Water Treatment Rule, in Federal Register. 2006, United States Environmental Protection Agency. p. 653–702.
  15. HMSO, The Water Supply (Water Quality) (Amendment) Regulations 199, in Statutory Instrument. 1999, Her Majesty’s Stationery Office: London.
  16. Haas CN. Epidemiology, microbiology, and risk assessment of waterborne pathogens including Cryptosporidium. J Food Prot. 2000;63(6):827–31.
  17. European Communitiy Directive 98/83/EC, Council Directive 98/83/EC of 3 November 1998 on the quality of water intended for human consumption as amended by Regulation 1882/2003/EC. 1998(31998L0083 (32003R1882)).
  18. LMG, Schweizerisches Bundesgesetz über Lebensmittel und Gebrauchsgegenstände (LMG), in 817.0. 1992.
  19. HyV, Schweizerische Hygieneverordnung des EDI vom 23. November 2005 (HyV), in 817.024.1. 2005.
  20. Smith HV, Smith PG. Parasitic protozoa in drinking water. Endeavour. 1990;14(2):74–9.
  21. Füchslin HP, Beuret C, Egli T. Mikrobiologische Belastung des Trinkwassers in Trinkwasserfassungen ländlicher Regionen. Gas, Wasser, Abwasser (GWA), 2005;11:859–65.
  22. SLMB, Schweizerisches Lebensmittelbuch. Schweizerische Eidgenossenschaft, 2000. Kapitel 56: Mikrobiologie.
  23. USEPA, Method 1623: Cryptospordium and Giardia in water by filtration/IMS/FA. EPA, 1999. 821-R-99-006(Office of Water).
  24. Wiedenmann A, Steuer S, Krüger P, Botzenhart K. A simple procedure for an exact evaluation of the sensitivity of the selective detection of viable Cryptosporidium oocysts by in vitro excystation and PCR. In: International Symposium on Waterborne Cryptosporidum Proceedings. 1997. Denver, Colorado, USA: AWWA.
  25. Ward PI, Deplazes P, Regli W, Rinder H, Mathis A. Detection of eight Cryptosporidium genotypes in surface and waste waters in Europe. Parasitol. 2002;124(Pt 4):359–68.
  26. Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutinary Genetics Analysis and sequence alignment. Briefings in Bioinformatics. 2004;5:150–63.
  27. Tatusova TA, Madden TL. Blast 2 sequences – a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett. 1999;174:247–50.
  28. Higgins D, Thompson J, Gibson T, Thompson JD, Higgins DG, Gibson TJ, CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:4673–80.
  29. Teunis P, Havelaar A. Cryptosporidium in drinking water: evaluation of the ILSI/RSI Quantitative Risk Assessment Framework. 1999, Rijksinstituut voor Volksgezondheid en Milieu RIVM: Bilthoven
  30. Zhou L, Fayer R, Trout JM, Ryan UM, Schaefer FW, 3rd, Xiao L. Genotypes of Cryptosporidium species infecting fur-bearing mammals differ from those of species infecting humans. Appl Environ Microbiol. 2004;70(12):7574–7.
  31. Schneider AT, Eberhardt R, Hartmann S, Herwig A, Heseker H, Hünchen K, et al. Ergebnisse der Nationalen Verzehrsstudie (1985–1988) über die Lebensmittel- und Nährstoffaufnahme in der Bundesrepublik Deutschland, in Band XI der VERA-Schriftenreihe. 1995, Wissenschaftlicher Fachverlag Dr. Fleck: Niederkleen.
  32. Auckenthaler A, Huggenberger P. Pathogene Mikroorganismen im Grund- und Trinkwasser. 2003, Basel: Birkhäuser Verlag.
  33. Lentze T, Hofer D, Gottstein B, Gaillard C, Busato A. Prevalence an importance of endoparasites in calves raised in Swiss cow-calf farms. Deutsche tierärztliche Wochenschrift. 1999;106:275–81.
  34. Nydam DV, Wade SE, Schaaf SL, Mohammed B. Number of Cryptosporidium parvum oocysts or Giardia spp. cysts shed by dairy calves after natural infection. Am J Vet Res. 2001;62(10):1612–5.
  35. Fretz R, Svoboda P, Ryan UM, Thompson RC, Tanners M, Baumgartner A. Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland. Epidemiol Infect. 2003;131(1):663–7.
  36. Morgan U, Weber R, Xiao L, Sulaiman I, Thompson RC, Ndiritu W, et al. Molecular characterization of Cryptosporidium isolates obtained from human immunodeficiency virus-infected individuals living in Switzerland, Kenya, and the United States. J Clin Microbiol. 2000;38(3):1180–3.
  37. Gläser C, Grimm F, Mathis A, Weber MD, Nadal D, Deplazes P. Detection and molecular characterization of Cryptosporidium spp. isolated from diarrhic children in Switzerland. Pediatr Infect Dis J. 2004;23(4):1–3.
  38. Baumgartner A, Marder HP, Munzinger J, Siegrist HH. Frequency of Cryptosporidium spp. as cause of human gastrointestinal disease in Switzerland and possible sources of infection. Schweiz Med Wochenschr. 2000;130(36):1252–8.
  39. Essers B, Burnens AP, Lanfranchini FM, Somaruga SG, von Vigier RO, Schaad UB, et al. Acute community-acquired diarrhea requiring hospital admission in Swiss children. Clin Infect Dis. 2000;31(1):192–6.
  40. Mäusezahl D, Egger M, Odermatt P, Tanner M. Clinical aspects and epidemiology of cryptosporidiosis in immunocompetent children. Schweiz Rundsch Med Prax. 1991;80:936–40.
  41. Weber R, Ledergerber B, Zbinden R, Altwegg M, Pfyffer GE, Spycher M, et al. Enteric infections and diarrhea in human immunodeficiency virus-infected persons: prospective community-based cohort study – Swiss HIV Cohort Study. Arch Intern Med. 1999;159:1473–80.
  42. Svoboda P, Ruchti S, Bissegger C, Tanner M. Occurence of Cryptosporidium spp. oocysts in surface, raw and drinking water samples. Mitt. Lebensm. Hyg. 1999;90(5):553–63.
  43. Regli W. Improved methods for the isolation and detection of Giardia-cysts and Cryptosporidium-oocysts form surface and drinking water: flocculation and FACS, in Vetsuisse Faculty of Zurich. 1994, University of Zurich, Switzerland: Zurich.
  44. Schweizer K. Modification and use of a method with FACS for the detection and quantification of Cryptosporidium oocysts in water, in Vetsuisse Faculty of Zurich. 1998, University of Zurich, Switzerland.
  45. Auckenthaler A, Raso G, Huggenberger P. Particle transport in a karst aquifer: natural and artificial tracer experiments with bacteria, bacteriophages and microspheres. Water Sci Technol. 2002;46(3):131–8.
  46. Chappell CL, Okhuysen PC, Sterling CR, Wang C, Jakubowski W, DuPont HL. Infectivity of Cryptosporidum parvum in healthy adults with pre-existing anti-C. parvum serum immunoglobulin G. Am J Trop Med Hyg. 1999;60(1):157–64.
  47. Frost FJ, Fea E, Gilli G, Biorci F, Muller TM, Craun GF, Calderon RL. Serological evidence of Cryptosporidium infections in southern Europe. Eur J Epidemiol. 2000;16(4):385–90.
  48. Harwood VJ, Levine AD, Scott TM, Chivukula V, Lukasik J, Farrah SR, Rose JB. Validity of the indicator organism paradigm for pathogen reduction in reclaimed water and public health protection. Appl Environ Microbiol. 2005;71(6):3163–70.
  49. Pell AN. Manure and microbes: public and animal health problem? J Dairy Sci. 1997;80(10):2673–81.