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Review article: Biomedical intelligence

Vol. 140 No. 3536 (2010)

Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier

  • B Rothen-Rutishauser
  • AD Lehmann
  • MJ Clift
  • F Blank
  • P Gehr
DOI
https://doi.org/10.4414/smw.2010.13060
Cite this as:
Swiss Med Wkly. 2010;140:w13060
Published
30.08.2010

Summary

A laser scanning microscope collects information from a thin, focal plane and ignores out of focus information. During the past few years it has become the standard imaging method to characterise cellular morphology and structures in static as well as in living samples. Laser scanning microscopy combined with digital image restoration is an excellent tool for analysing the cellular cytoarchitecture, expression of specific proteins and interactions of various cell types, thus defining valid criteria for the optimisation of cell culture models. We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall.

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